Oncotarget

Research Papers:

Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo

Moammir H. Aziz, Xundi Chen, Qi Zhang, Chad DeFrain, Jared Osland, Yizhou Luo, Xin Shi, Rong Yuan _

PDF |  HTML  |  Supplementary Files  |  Author Information  |  Order a Reprint

Oncotarget. 2015; 6:39714-39724. https://doi.org/10.18632/oncotarget.5356

Metrics: HTML 243 views  |   ?  


Abstract

Moammir H. Aziz1, Xundi Chen2, Qi Zhang3, Chad DeFrain4, Jared Osland1, Yizhou Luo5, Xin Shi3, Rong Yuan1,2

1Division of Geriatrics, Department of Internal Medicine, Southern Illinois University School of Medicine, Springfield, Illinois 62794-9628, USA

2Department of Medical Microbiology and Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, Illinois 62794-9628, USA

3Zhongda Hospital, Southeast University of China, Nanjing, China 210009

4Department of Pathology, Southern Illinois University School of Medicine, Springfield, Illinois 62794-9628, USA

5Department of Oncology, Nanjing Junxie Hospital, Nanjing, China, 210002

Correspondence to:

Rong Yuan, e-mail: ryuan@siumed.edu

Keywords: NRIP1, breast cancer, human tissue array, apoptosis, cell survival

Received: April 13, 2015     Accepted: October 03, 2015     Published: October 15, 2015

ABSTRACT

Earlier age at menarche is a major risk factor for breast cancer. Our previous study identified Nrip1 (also known as Rip140) as a candidate gene for delaying female sexual maturation (FSM) and found that knocking out Nrip1 could significantly delay FSM in mice. To investigate the effects of NRIP1 in breast cancer we used human cell lines and tissue arrays along with an in vivo study of DMBA-induced carcinogenesis in Nrip1 knockout mice. Analysis of tissue arrays found that NRIP1 is elevated in tumors compared to cancer adjacent normal tissue. Interestingly, in benign tumors NRIP1 levels are higher in the cytosol of stromal cells, but NRIP1 levels are higher in the nuclei of epithelial cells in malignancies. We also found overexpression of NRIP1 in breast cancer cell lines, and that suppression of NRIP1 by siRNA in these cells significantly induced apoptosis and inhibited cell growth. Furthermore, in vivo data suggests that NRIP1 is upregulated in DMBA-induced breast cancer. Importantly, we found that DMBA-induced carcinogenesis is suppressed in Nrip1 knockdown mice. These findings suggest that NRIP1 plays a critical role in promoting the progression and development of breast cancer and that it may be a potential therapeutic target for the new breast cancer treatments.

Author Information

Moammir H. Aziz

Xundi Chen

Qi Zhang

Chad DeFrain

Jared Osland

Yizhou Luo

Xin Shi

Rong Yuan
Primary Contact  _


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 5356