Oncotarget

Research Papers:

RhoE is required for contact inhibition and negatively regulates tumor initiation and progression

Marta Hernández-Sánchez, Enric Poch, Rosa M. Guasch, Joaquín Ortega, Inmaculada López-Almela, Ignacio Palmero, Ignacio Pérez-Roger _

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Oncotarget. 2015; 6:17479-17490. doi: 10.18632/oncotarget.4127

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Abstract

Marta Hernández-Sánchez1,5,*, Enric Poch1,*, Rosa M. Guasch2, Joaquín Ortega3, Inmaculada López-Almela1, Ignacio Palmero4 and Ignacio Pérez-Roger1

1 Universidad CEU-Cardenal Herrera, Facultad de Ciencias de la Salud, Dep Ciencias Biomédicas, Moncada, Spain

2 Centro de Investigación Príncipe Felipe, Rho Signaling in Neuropathologies, Valencia, Spain

3 Universidad CEU-Cardenal Herrera, Facultad de Veterinaria, Dep. PASACTA, Moncada, Spain

4 Instituto de Investigaciones Biomédicas “Alberto Sols” CSIC-UAM, Madrid, Spain

5 Departament de Biologia cellular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallés, Spain

* These authors have contributed equally to this work and should therefore be considered equal first authors

Correspondence to:

Ignacio Pérez-Roger, email:

Ignacio Palmero, email:

Keywords: contact inhibition, metastasis, RhoE, tumor suppression, p27Kip1

Received: November 01, 2014 Accepted: May 02, 2015 Published: May 12, 2015

Abstract

RhoE is a small GTPase involved in the regulation of actin cytoskeleton dynamics, cell cycle and apoptosis. The role of RhoE in cancer is currently controversial, with reports of both oncogenic and tumor-suppressive functions for RhoE. Using RhoE-deficient mice, we show here that the absence of RhoE blunts contact-inhibition of growth by inhibiting p27Kip1 nuclear translocation and cooperates in oncogenic transformation of mouse primary fibroblasts. Heterozygous RhoE+/gt mice are more susceptible to chemically induced skin tumors and RhoE knock-down results in increased metastatic potential of cancer cells. These results indicate that RhoE plays a role in suppressing tumor initiation and progression.

Author Information

Marta Hernández-Sánchez

Enric Poch

Rosa M. Guasch

Joaquín Ortega

Inmaculada López-Almela

Ignacio Palmero

Ignacio Pérez-Roger
Primary Contact  _


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