Oncotarget

Research Papers:

Global 5-hydroxymethylcytosine content is significantly reduced in tissue stem/progenitor cell compartments and in human cancers

Michael Christoph Haffner, Alcides Chaux, Alan K Meeker, David Esopi, Jonathan Gerber, Laxmi G. Pellakuru, Antoun Toubaji, Pedram Argani, Christine Iacobuzio-Donahue, William G. Nelson, George J. Netto, Angelo DeMarzo, Srinivasan Yegnasubramanian _

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Abstract

Michael C. Haffner1, Alcides Chaux2, Alan K. Meeker1,2,3, David M. Esopi1, Jonathan Gerber4, Laxmi G. Pellakuru2, Antoun Toubaji2, Pedram Argani1,2, Christine Iacobuzio-Donahue1,2, William G. Nelson1,2,3, George J. Netto1,2,3, Angelo M. De Marzo1,2,3, Srinivasan Yegnasubramanian1

1 Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, Maryland, USA

2 Department of Pathology, Johns Hopkins University, Baltimore, Maryland, USA

3 Brady Urological Institute, Johns Hopkins University, Baltimore, Maryland, USA

4 Department of Medicine, Division of Hematology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA

Received: September 1, 2011; Accepted: September 1, 2011; Published: September 2, 2011;

Keywords: 5-hydroxymethylcytosine, 5hmC, DNA methylation, differentiation, cancer, tissue stem / progenitor cells

Correspondence:

Srinvasan Yegnasubramanian, email:

Abstract

DNA methylation at the 5-position of cytosines (5mC) represents an important epigenetic modification involved in tissue differentiation and is frequently altered in cancer. Recent evidence suggests that 5mC can be converted to 5-hydroxymethylcytosine (5hmC) in an enzymatic process involving members of the TET protein family. Such 5hmC modifications are known to be prevalent in DNA of embryonic stem cells and in the brain, but the distribution of 5hmC in the majority of embryonic and adult tissues has not been rigorously explored. Here, we describe an immunohistochemical detection method for 5hmC and the application of this technique to study the distribution of 5hmC in a large set of mouse and human tissues. We found that 5hmC was abundant in the majority of embryonic and adult tissues. Additionally, the level of 5hmC closely tracked with the differentiation state of cells in hierarchically organized tissues. The highest 5hmC levels were observed in terminally differentiated cells, while less differentiated tissue stem/progenitor cell compartments had very low 5hmC levels. Furthermore, 5hmC levels were profoundly reduced in carcinoma of the prostate, breast and colon compared to normal tissues. Our findings suggest a distinct role for 5hmC in tissue differentiation, and provide evidence for its large-scale loss in cancers.

Author Information

Michael Christoph Haffner
Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University, Baltimore, Maryland, 21231 USA

Alcides Chaux
Department of Pathology Johns Hopkins University, Baltimore, Maryland, 21231 USA

Alan K Meeker
Department of Pathology Johns Hopkins University, Baltimore, Maryland, 21231 USA

David Esopi
Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University, Baltimore, Maryland, 21231 USA

Jonathan Gerber
Department of Medicine, Division of Hematology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, 21231 USA

Laxmi G. Pellakuru
Department of Pathology Johns Hopkins University, Baltimore, Maryland, 21231 USA

Antoun Toubaji
Department of Pathology Johns Hopkins University, Baltimore, Maryland, 21231 USA

Pedram Argani
Department of Pathology Johns Hopkins University, Baltimore, Maryland, 21231 USA

Christine Iacobuzio-Donahue
Department of Pathology Johns Hopkins University, Baltimore, Maryland, 21231 USA

William G. Nelson
Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University, Baltimore, Maryland, 21231 USA

George J. Netto
Department of Pathology Johns Hopkins University, Baltimore, Maryland, 21231 USA

Angelo DeMarzo
Department of Pathology Johns Hopkins University, Baltimore, Maryland, 21231 USA

Srinivasan Yegnasubramanian
Primary Contact  _

Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University, Baltimore, Maryland, 21231 USA


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