Oncotarget

Research Papers:

SIRPα-antibody fusion proteins stimulate phagocytosis and promote elimination of acute myeloid leukemia cells

Laia Pascual Ponce, Nadja C. Fenn, Nadine Moritz, Christina Krupka, Jan-Hendrik Kozik, Kirsten Lauber, Marion Subklewe, Karl-Peter Hopfner _

Metrics: PDF 689 views  |   HTML 332 views  |   ?  


Abstract

Laia Pascual Ponce1,5, Nadja C. Fenn1, Nadine Moritz1, Christina Krupka2,3, Jan-Hendrik Kozik1, Kirsten Lauber4, Marion Subklewe2,3, Karl-Peter Hopfner1,5

1Gene Center Munich, Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany

2Department of Internal Medicine III, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

3Gene Center and Clinical Co-operation Group Immunotherapy at the Helmholtz Zentrum München, Munich, Germany

4Department of Radiation Oncology, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

5Graduate School of Quantitative Biosciences Munich, Ludwig-Maximilians-Universität München, Munich, Germany

Correspondence to:

Karl-Peter Hopfner, email: hopfner@genzentrum.lmu.de

Keywords: therapeutic antibody, immunotherapy, CD47, SIRPα, acute myeloid leukemia

Received: August 02, 2016     Accepted: December 12, 2016     Published: January 04, 2017

ABSTRACT

CD47, expressed on a variety of tumor cells, confers immune resistance by delivering an inhibitory “don’t eat me” signal to phagocytic cells via its myeloid-specific receptor SIRPα. Recent studies have shown that blocking the CD47-SIRPα axis with CD47-directed antibodies or antibody-derivatives enhances phagocytosis and increases antitumor immune effects. However, CD47 expression on healthy cells creates an antigen sink and potential sites of toxicity, limiting the efficacy of CD47-directed therapies. In this study, we first characterized CD47 expression in Acute Myeloid Leukemia (AML) patients (n = 213) and found that CD47 is highly expressed on both AML bulk and stem cells irrespective of the disease state. Furthermore, to inhibit the CD47-SIRPα signaling pathway at the tumor site, we developed a so-called local inhibitory checkpoint monoclonal antibody (licMAB) by grafting the endogenous SIRPα domain to the N-terminus of the light chain of an antibody targeting CD33, a surface antigen expressed in AML. LicMABs selectively bind CD33-expressing cells even in the presence of a large CD33-negative CD47-positive antigen sink, stimulate phagocytosis of AML cells and eliminate AML cell lines and primary, patient-derived AML cells. Our findings qualify licMABs as a promising therapeutic approach to confine the benefit of disrupting the CD47-SIRPα axis to tumor antigen-expressing cells.

Author Information

Laia Pascual Ponce

Nadja C. Fenn

Nadine Moritz

Christina Krupka

Jan-Hendrik Kozik

Kirsten Lauber

Marion Subklewe

Karl-Peter Hopfner
Primary Contact  _


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 14500