Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Variation of lifespan in multiple strains, and effects of dietary restriction and BmFoxO on lifespan in silkworm, Bombyx mori

Jiangbo Song, Dongmei Tang, Zhiquan Li, Xiaoling Tong, Jianfei Zhang, Minjin Han, Hai Hu, Cheng Lu, Fangyin Dai _

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Abstract

Jiangbo Song1,2,*, Dongmei Tang1,2,*, Zhiquan Li1,2,*, Xiaoling Tong1,2, Jianfei Zhang1,2, Minjin Han1,2, Hai Hu1,2, Cheng Lu1,2 and Fangyin Dai1,2

1 State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China

2 Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China

* These authors are co-first authors

Correspondence to:

Fangyin Dai, email:

Keywords: silkworm, lifespan, dietary restriction, BmFoxO, experimental animal, Gerotarget

Received: September 27, 2016 Accepted: December 20, 2016 Published: December 26, 2016

Abstract

Established animal models have accelerated our understanding of the mechanisms involved in lifespan determination. However, more experimental animals are required to clarify the complex mechanisms behind the phenomena of aging and lifespan. In this study, we reported the variation of lifespan in nine distinct silkworm strains. Lifespan correlated significantly with BmFoxO gene expression in the representative silkworm strains tested (Xiafang, Dazao-N, and N4). In general, the female silkworm was longer lived than the male of the same strain. Dietary restriction extended the silkworm lifespan compared with that of silkworms fed ad libitum. The expression of BmFoxO was significantly elevated in the dietary restriction group on day 3 of the 4th instar and day 3 of the 5th instar, suggesting that BmFoxO contributes to dietary restriction-mediated lifespan extension. The RNA interference and overexpression of the BmFoxO gene significantly shortened and extended the silkworm adulthood, respectively. In conclusion, our findings suggest that the silkworm might serve as a promising experimental animal to explore the complex biological mechanisms of lifespan determination.

Author Information

Jiangbo Song

Dongmei Tang

Zhiquan Li

Xiaoling Tong

Jianfei Zhang

Minjin Han

Hai Hu

Cheng Lu

Fangyin Dai
Primary Contact  _


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