Oncotarget

Research Papers:

The tri-peptide GHK-Cu complex ameliorates lipopolysaccharide-induced acute lung injury in mice

Jeong-Ran Park, Hanbyeol Lee, Seok-In Kim, Se-Ran Yang _

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Oncotarget. 2016; 7:58405-58417. doi: 10.18632/oncotarget.11168

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Abstract

Jeong-Ran Park1,2, Hanbyeol Lee1, Seok-In Kim3, Se-Ran Yang1

1Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea

2Institute of Medical Science, Kangwon National University, Chuncheon, Republic of Korea

3Bioceltran Co., Ltd., Chuncheon, Republic of Korea

Correspondence to:

Se-Ran Yang, email: seran.yang@gmail.com, seran@kangwon.ac.kr

Keywords: GHK-Cu, acute lung injury, inflammation, NF-κB p65, p38 MAPK

Received: April 28, 2016    Accepted: July 28, 2016    Published: August 10, 2016

ABSTRACT

The tripeptide-copper complex glycyl-l-histidyl-l-lysine-Cu (II) (GHK-Cu) is involved in wound healing and tissue remodeling. Although GHK-Cu exhibits anti-aging and tissue renewing properties, its roles in acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) are still unknown. Therefore, we examined the effects of GHK-Cu in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages in vitro and ALI in mice in vivo. GHK-Cu treatment reduced reactive oxygen species (ROS) production, increased superoxide dismutase (SOD) activity while decreased TNF-α and IL-6 production through the suppression of NF-κB p65 and p38 MAPK signaling in vitro and in vivo model of ALI. Moreover, GHK-Cu attenuated LPS-induced lung histological alterations, suppressed the infiltration of inflammatory cells into the lung parenchyma in LPS-induced ALI in mice. Taken together, these findings demonstrate that GHK-Cu possesses a protective effect in LPS-induced ALI by inhibiting excessive inflammatory responses; accordingly it may represent a novel therapeutic approach for ALI/ARDS.

Author Information

Jeong-Ran Park

Hanbyeol Lee

Seok-In Kim

Se-Ran Yang
Primary Contact  _


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