Extracellular matrix composition modulates angiosarcoma cell attachment and proliferation

Noel L. Shaheen1,Esha Kataria2, Jocelyn Antony1, Dana Galvan1, Yessenia Ballou2, Brad A. Bryan1,2

1 Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX, USA

2 Department of Biomedical Sciences, Texas Tech University Health Sciences Center, El Paso, TX, USA

Correspondence to:

Brad A. Bryan, email: brad.bryan@ttuhsc.edu

Keywords: angiosarcoma, extracellular matrix, fibronectin, collagen, endothelial

Received: June 02, 2017 Accepted: November 11, 2017 Published: December 07, 2017


Introduction: Angiosarcoma is a rare and generally fatal tumor composed of aberrant cells of endothelial origin. Because of its infrequency in humans, very little is known about the growth requirements of this vascular sarcoma. Unlike the rapidly proliferating solid tumors from which they are isolated from, many of the established angiosarcoma cell lines exhibit less than robust growth in culture and often fail to form tumors in xenograft models. In order to better understand angiosarcoma in vitro growth conditions, we focuse on a singular aspect of their culture--adhesion to the extracellular matrix-- in order to identify attachment substrates that may facilitate and/or enhance their growth in tissue culture. Our data indicates that the extracellular matrix of angiosarcomas contains similar protein compositions to that of non-diseased endothelial cells. Moreover, angiosarcoma cell lines exhibited strong attachment preference to substrates such as collagen I or fibronectin, and less preference to collagen IV, laminin, or tropoelastin. Growth on preferred extracellular matrix substrates promoted mitogenic signaling and increased proliferation of angiosarcoma cell lines. These findings provide insight that may lead to more successful in vitro growth of angiosarcoma cell lines.

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